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Medical Laboratory Technician Skills Checklist

Please rate your experience / frequency within the last year

0 = No Experience / Observed Only

1 = Limited Experience / Rarely Done (<6 times/year)

2 = May Need Some Review / Occasionally Done (1 - 2 times/month)

3 = Experienced / Frequently Done (daily or weekly)

AGE OF PATIENTS CARED FOR

Newborn/Neonate (birth to 30 days)
Infant (31 days to 12 months)
Toddler/Preschool (13 months to 5 years)
School Age Child/adolescent (6 years to 18 years)
Young Adults/Middle Adult (19 years to 64 years)
Older Adults/Elderly (65+ years)

EXPERIENCE

Hospital Laboratory
Clinic Experience
Blood Bank
Hematology
Chemistry
Body Fluid Testing
Coagulation
Immunology/Serology
Microbiology
Basic Lab Procedures
Basic Lab Safety
Sources of Error in Lab Testing
Quality Assurance in Lab Testing
Decontamination Procedures
Knowledge of "Do Not Use Abbreviations"
Do you have charge/supervisor experience?
Knowledge of current Joint Commission National Patient Safety Goals
Knowledge/familiarity with HCAHPS scores

Selecting Appropriate

Type of sample for test requested
Methods for test requested
Reagents for test
Media for test
Control for test
Instrument to perform test
Routine procedures to verify results
Special procedures to verify results

Preparing Appropriate

Samples
Reagents
Media
Blood Products
Controls
Instruments
Accurately calculating results of testing
Verify Test Results for reporting
Recognize Inconsistent results
Recognize sources of error
Check for common procedural problems
Check for common technical problems
Take corrective action
Determine Instrument adjustments
Assess accuracy of test procedures
Recognize abnormal results
Recognize the need for additional testing

Phlebotomy

Finger stick
Heel stick
Butterfly
Venipuncture
Pediatric
Knowledge of tube types and uses
Manual Pipetting

General Laboratory Practice

Glucose tolerance testing
Glucometer use

Hematology

Automated CBC
Differential
Manual Pipetting
Automated CBC
RBC morphology
WBC morphology
Platelet morphology
Manual cell counts
RBC Indices
Tests for Hemoglobin defects
Fetal hemoglobin
ANC ( Absolute Neutrophil Count)
Bone Marrow Preparation
CSF
Cell count
Differential
Retic count
ESR (Manual or automated)
Body Fluid Cell Count
Sickle Cell prep
Special stains (PAS,Peroxidase, Leukocyte Aldaline Phospatase)
Scattergram interpretation
Histogram interpretation
Knowledge of alert and panic values

Instruments used for Hematology:

Coagulation

PT,PTT,INR
Circulating Anticoagulants
Thrombin Time
Fibrinogen
Bleeding Times (Ivy and Duke)

Blood Bank

ABO testing
RH testing
Cross match - tube method
Cross match - gel method
Compatibility testing
Transfusion practices/indications
Patient identification
Issuing blood components
Emergency release
Antibody screen
Antibody identification
Antibody titer
Blood irradiation
Cell packing
Cell washing
Plasmapheresis
Plateletpheresis
Immediate spin cross match
Freezing blood
Thawing procedures for platelets
Thawing procedures for FFP
RBC's
Platelets
FFP
Cryoprecipitate
Cord blood
Rhogam
Rh IgG
Coombs Test direct
Coombs test indirect
Transfusion adverse reaction
Special tests (DAT,Phenotyping, etc.)

Instruments used for Blood Bank:

Chemistry

General chemistry
BMP
CMP
Immunoassay chemistry
Lipid Profile
CRP
Immunoelectropheresis
Iron and TIBC
Acetone analysis s (serum and urine)
Ammonia
Osmolality
Blood gases
Blood gases
Drug screening
Gas chromatography
Thin layer chromatography
Blood alcohol level
Electropheresis
Immunoelectropheresis
Immunoassay chemistry
Tumor markers
Therapeutic drug levels
Toxicology
ISE procedures
CSF Protein
Knowledge of alert and panic values

Instruments used for Chemistry:

Urinalysis

Manual UA
Automated UA
Microscopic UA
24 hour urine collection
Urine pregnancy test
Semen analysis
Crystal identification
Urine drug testing
Chain of Custody documentation
Knowledge of alert and panic values

Instrumentation used for Urinalysis:

Immunology/Serology

ANA
ASO Titer
CMV
C3,C4, Total complement
Rubella serology
MMR serology
Hepatitis
HIV
Influenza screen A and B
Meningitis screen
Monospot
Rapid strep
Rheumatoid factor
Pertussis
pregnancy testing/HCG
RPR
Cold agglutinins
Febrile agglutinins
RSV
H Pylori
LE Cell Prep (Lupus)
Mycoplasma
Nephelometry
PCR technology
T and B cell studies
TORCH studies
ELISA testing
Western Blot Technology
Autoimmunity
Cellular and Humoral
DNA Probe Technology
Flow cytometry
FTA-ABS
Hemagglutination inhibition
Hormone testing
Radial immuno diffusion
Knowledge of alert values

Instrumentation used for Immunology/Serology:

Microbiology

Aerobic culture
Anaerobic culture
Aerobic gram positive cocci
Gram stain
Gram negative cocci
Gram positive cocci
Gram negative bacilli
Gram positive bacilli
Blood culture
Cell culture
Viral cultures
Virus identification
Virus isolation
Fungal culture
Chlamydia culture
Chlamydia smear
CO2 cultures
Mycobacteria culture
culture plating
Acid fast stain (fluorescent)
Acid fast stain (Kinyoun)
Malarial exam
sensitivities (KB and MIC)
Organism identification
India Ink
KOH prep
Prep and ID
Occult blood
C difficil assay
Dark field
wet mount
Knowledge of alert values

Instrumentation used for Microbiology:

Histology

Concept of Fixation
Assist with frozen sectioning
Assist with Gross Sectioning
QC Req and Documentation
Collection of Tissue Specimen
Bone Marrow Biopsy and Slides
Embed Tissue (incl small Bxs)
Running of Tissue Processor
Microtome and Disposable Blades
Filling ofSlides and Block
Principle of Hematoxylin and Eosin Stain
Decalcify Bone and Hand Tissue
Grossing Small Specimen
Crypto-spin preps
Immuno stains
Special Stain Techniques
Group I
Group II
Group III
Group IV
Group V
Storage Time of Wet Tissues
Disposal of Wet Tissues
Universal Histology Precautions
Coverslipping
Preparation of Stains/Reagents
Proper Labeling of Reagants
MOHS Sectioning
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